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RNase Control

There many applications in which it is important to maintain the integrity of RNA. NEB offers three separate RNase inhibitors; Ribonucleoside Vanadyl Complex (NEB #S1402) can be used in cell fractionation methods, as well as in sucrose-gradient centrifugation to inhibit various ribonucleases; while RNase Inhibitor, Human Placenta (NEB #M0307) or RNase Inhibitor, Murine (NEB #M0314) can be used for inhibition of RNases A, B and C. The advantage of RNase Inhibitor, Murine, is that it lacks a pair of easily-oxidized cysteines that are present in the human form, thereby making it more stable.


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Protocols for RNase Control

Reagents for RNA Sample Preparation

NEB offers a wide variety of reagents to facilitate front-end processing of RNA for downstream applications including next-generation sequencing, library construction and microarrays.

RNA Polymerase Selection Chart

NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.

RNA Ligase Selection Chart

NEB offers a variety of ligases for RNA research with unique specificities.

cDNA Synthesis Selection Chart

NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.