RNA Reagents
RNA plays a multitude of cellular roles in all kingdoms of life. RNA is an essential carrier of genetic information that can catalyze chemical reactions and influence gene expression.
In the last several years, our understanding of RNAs as regulatory molecules in the cell has dramatically changed. Many new classes of small and large non-coding RNAs with largely unexplored functions have been recently reported, ushering in a renaissance of RNA-focused research in biology.
NEB offers a range of products for RNA research, including reagents for synthesis, extraction, purification, cleanup, analysis, modification and detection of RNA molecules.
RNA Reagents includes these subcategories:
FAQs for RNA Reagents
Protocols for RNA Reagents
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Latest NEB Expressions
This edition of NEB expressions focuses on tools that can streamline your RNA-related workflows. Featured products include NEBNext Ultra II kits for RNA, the Monarch Total RNA Miniprep Kit, Luna reagents for RT-qPCR, the new LunaScript RT SuperMix Kit and HiScribe kits for high yield, high quality RNA synthesis. Learn more about functional considerations for RNA synthesis in our feature, and learn about a new protocol for Cappable Seq. Lastly, read about ways you can promote sustainable science in the lab.
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RNA Metro Map
Download our RNA poster to learn more about the various RNA structures and recent applications.
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RNA Synthesis Brochure
Learn more about NEB's products for RNA synthesis, which range from template generation to poly(A) tailing.
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RNA Technical Guide
Find in depth information on tools designed to streamline your RNA workflows, including RNA extraction & purification, qPCR and RT-qPCR, RNA-seq, RNA synthesis and gRNA synthesis.
- Recommended HiScribe RNA Synthesis Kits by Application
- Troubleshooting Guide for RNA Cleanup
- Troubleshooting Guide for Total RNA Extraction & Purification
- Avoiding Ribonuclease Contamination
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
Other Tools & Resources
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Fuchs, R.T., Sun, Z., Zhuang, F., et al. 2015. Bias in ligation-based small RNA sequencing library construction is determined by adaptor and RNA structure PLoS One. 10(5), PubMedID: 25942392, DOI: 10.1371/journal.pone.0126049
Publications related to RNA Reagents
RNA Reagents Overview
Reagents for RNA Sample Preparation
RNA Polymerase Selection Chart
RNA Ligase Selection Chart
cDNA Synthesis Selection Chart
Legal Information
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Videos
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In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene.
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Tips for Successful RNA Purification using Monarch Total RNA Miniprep Kit
Optimize the results of your RNA extractions with these simple tips from our scientists.
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Monarch Total RNA Miniprep Protocol
This video walks you through RNA purification from a wide variety of sample types using the Monarch Total RNA Miniprep Kit.
