BsaI-HF^®v2 has been optimized for Golden Gate Assembly. BsaI-HFv2 also works well for any protocol requiring DNA cutting by BsaI. This is the recommended enzyme for any purpose requiring digestion at the recognition sequence: 5′-GGTCTC(N1)/(N5)-3′.

High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Product Source

This product is related to the following categories:

High-Fidelity (HF®) Restriction Endonucleases Products,

Restriction Endonucleases B Products,

Time-Saver Qualified Restriction Enzymes Products

This product can be used in the following applications:

Restriction Enzyme Digestion,

NEBridge® Golden Gate Assembly ,

Fast Cloning: Accelerate your cloning workflows with reagents from NEB,

Restriction Enzyme Digestion