BsaI-HF®v2
cloned at NEB recombinant engineered time saver neb31 dil_B 37 80 Heat CpG dcm

Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (35+ fragments).

Inquire about BSA-free formulations

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at www.neb.com/BSA-free.

BsaI-HFv2 is NEB Type IIS restriction enzyme that has been optimized for Golden Gate Assembly and in protocols requiring DNNEB cutting by BsaI Works well for any protocol requiring DNNEB cutting by BsaI Recommended enzyme for any purpose requiring digestion at the recognition sequence 5-GGTCTC(N1)/(N5)-3

Attention Golden Gate Assembly users: BsaI-HFv2 (NEB #R3733) has been optimized for Golden Gate Assembly*.  BsaI-HFv2 also works well for any protocol requiring DNA cutting by BsaI. This is the recommended enzyme for any purpose requiring digestion at the recognition sequence ...5′-GGTCTC(N1)/(N5)-3′...
 
*The requirements for digestion of DNA during Golden Gate Assembly are more demanding than what is encountered in traditional DNA cloning. For Golden Gate, the enzyme must function well in a buffer that might not be the same buffer as that normally provided for the restriction enzyme, and maintain activity for an extended time at elevated temperatures in a dynamic cutting/re-ligating reaction with competition for substrate binding between the endonuclease and ligase. Extensive testing has demonstrated superior performance of BsaI-HFv2 compared to both BsaI (NEB #R0535) and BsaI-HF (NEB #R3535) in this challenging Golden Gate assembly context where restriction enzyme efficiency and fidelity are critically important.

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart™ Buffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

  • Engineered for improved performance
  • Used in Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer often used in this application
  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • 100% activity in rCutSmart Buffer
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • Reduced star activity
  • NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly.
  • Cut Site: GGTCTC(1/5)

"I really, really love BsaI-HFv2. We are back to getting phenomenal GGA results again with very little background."

    -M.C, Principal Investigator, Davidson College, NC

Catalog # Concentration Size
R3733S Not Applicable 1,000 units
R3733L Not Applicable 5,000 units
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