HindIII-HF®
cloned at NEB recombinant engineered time saver neb31 dil_B 37 80 Heat

HindIII-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10119499. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB high fidelity restriction endonuclease that recognizes the sequence A^AGCT_T

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmartBuffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

  • Engineered for improved performance
  • 100% activity in rCutSmart Buffer
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • Reduced star activity
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X)
  • Restriction Enzyme Cut Site: A/AGCTT
Catalog # Concentration Size
R3104S 20,000 units/ml 10,000 units
R3104T 100,000 units/ml 10,000 units
R3104L 20,000 units/ml 50,000 units
R3104M 100,000 units/ml 50,000 units
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