BsmBI-v2

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
R0739S     -20       BsmBI-v2 R0739SVIAL -20 1 x 0.02 ml 10,000 units/ml   NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
R0739L     -20       BsmBI-v2 R0739LVIAL -20 1 x 0.1 ml 10,000 units/ml   NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Companion Products

• NEBridge^® Golden Gate Assembly Kit (BsaI-HF^®v2)
• Esp3I
• r3733-bsaI-hf-v2
• BbsI-HF^®
• Nuclease-free Water

Materials Sold Separately

• Gel Loading Dye, Purple (6X), no SDS

1. BsmBI-v2 is an isoschizomer of Esp3I

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes
4. 35-Fragment Golden Gate Assembly using BsmBI-v2 (NEB #E1602)
5. Golden Gate Assembly Protocol for using NEBridge^® Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602)

• Double Digests
• Expanded “assembly standards” for MoClo, GoldenBraid2.0 and other modular Golden Gate Assembly methods
• Optimizing Restriction Endonuclease Reactions

• Alphabetized List of Recognition Sequences
• Cleavage of Supercoiled DNA
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Dam-Dcm and CpG Methylation
• Enzymes with Nonpalindromic Sequences
• Frequencies of Restriction Sites
• Isoelectric Points (pI) for Restriction Enzymes
• Isoschizomers
• NEB Diluent and Buffer Table
• Time-Saver™ Qualified Enzymes
• Type IIS Restriction Enzymes
• Why Choose Recombinant Enzymes?

• DNA Sequences and Maps Tool

• Double Digest Finder
• Enzyme Finder
• NEBridge^™ Golden Gate Assembly Tool
• NEBcutter™ v3.0
• NEBioCalculator®
• REBASE®

1. Why is there a BsmBI site in LITMUS 38 but not LITMUS 39?
2. Which restriction enzymes are used in Golden Gate Assembly?
3. Which restriction enzymes are used in GoldenBraid Assembly?
4. When is star activity a concern?
5. Is Gel Loading Dye, Purple (6X) or Gel Loading Dye, Purple (6X), no SDS compatible with other DNA binding dyes such as SYBR® and GelRed™ during gel electrophoresis?
6. Why is my Restriction Enzyme not cutting DNA?
7. Why do I see additional DNA bands on my gel after a restriction digest?
8. Why do I see a DNA smear on an agarose gel after a restriction digest?
9. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
10. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
11. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
12. What are the differences between BsmBI-v2 and its isoschizomer, Esp3I?

• Golden Gate Assembly brochure

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0739S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0739L_v1_10059568
• R0739S_v1_10059567
• R0739S_v1_10069104
• R0739S_v1_10072879
• R0739L_v1_10072878
• R0739L_v1_10076651
• R0739S_v1_10077760
• R0739L_v1_10078100
• R0739L_v1_10079772
• R0739S_v1_10079011
• R0739S_v1_10080281
• R0739L_v1_10081461
• R0739L_v1_10081486
• R0739S_v1_10081485
• R0739S_v1_10084573
• R0739S_v1_10088076
• R0739L_v1_10088077
• R0739S_v1_10091130
• R0739S_v1_10093310
• R0739L_v1_10095228
• R0739L_v1_10097393
• R0739S_v1_10097293
• R0739L_v1_10099764
• R0739S_v1_10099762
• R0739S_v1_10105217
• R0739L_v1_10107035
• R0739S_v1_10111530
• R0739L_v1_10114601
• R0739S_v1_10118457
• R0739L_v1_10119854
• R0739S_v1_10123721
• R0739L_v1_10128738
• R0739S_v1_10131499
• R0739L_v1_10133500
• R0739S_v1_10137513
• R0739L_v1_10139502
• R0739L_v1_10139898
• R0739S_v1_10139896
• R0739S_v1_10147202
• R0739L_v1_10149877
• R0739S_v1_10151161
• R0739S_v1_10156008
• R0739L_v1_10154043
• R0739L_v1_10160776
• R0739S_v1_10160208
• R0739L_v1_10165084
• R0739S_v1_10165083
• R0739S_v1_10170459
• R0739L_v1_10170457
• R0739S_v1_10173614
• R0739L_v1_10176972
• R0739S_v1_10180154
• R0739L_v1_10180155
• R0739S_v1_10185798

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• BsmBI-v2
• NEBuffer™ r3.1
• Gel Loading Dye, Purple (6X)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.