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BspQI

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_

Isoschizomers | Single Letter Code | Pronunciation:

Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.

Time-Saver™ qualified for digestion in 5-15 minutes
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Cut Site: GCTCTTC(1/4)

Catalog #	Concentration	Size
R0712S	10,000 units/ml	500 units
R0712L	10,000 units/ml	2,500 units
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Product Information

Product Source

An E. coli strain that carries the cloned BspQI gene from Bacillus sphaericus (X.S. Pan)

Reagents Supplied

The following reagents are supplied with this product:

Show all

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0712S -20

BspQI R0712SVIAL -20 1 x 0.05 ml 10,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

R0712L -20

BspQI R0712LVIAL -20 1 x 0.25 ml 10,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Product Categories:: Restriction Endonucleases B,; time-saver-qualified-restriction-enzymes Products

Applications:: Golden Gate Assembly,; Fast Cloning: Accelerate your cloning workflows with reagents from NEB,; Restriction Enzyme Digestion

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 50°C in a total reaction volume of 50 µl.
Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 50°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl₂
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 100%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent B
Storage Buffer

20 mM Tris-HCl
500 mM KCl
1 mM DTT
0.1 mM EDTA
500 µg/ml BSA
50% Glycerol
0.1% Triton® X-100
pH 7 @ 25°C

Heat Inactivation
80°C for 20 min
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Isoschizomers

LguI
PciSI
SapI

Activity at Temperature
@37°C: 50%
Related Products
Companion Products
Materials Sold Separately
- NEBuffer™ r3.1
Product Notes
1. Based on the stability of the enzyme in the reaction, incubations longer than 1 hr will not result in improved digestion, unless additional enzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
2. Star activity may result in a low salt buffer, such as rCutSmart Buffer. For an optimal restriction digest protocol please see NEBCloner.
3. Not sensitive to CpG, dcm, or dam methylation.
4. star activity may result from a glycerol concentration of >5%