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BspQI

GMP-grade reagent also available. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_

Isoschizomers | Single Letter Code | Pronunciation:

Linearize plasmid template before in vitro transcription
Time-Saver™ qualified for digestion in 5-15 minutes
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Restriction Enzyme Cut Site: GCTCTTC(1/4)

Linearize plasmid template before in vitro transcription
Time-Saver™ qualified for digestion in 5-15 minutes
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Restriction Enzyme Cut Site: GCTCTTC(1/4)

* "GMP-grade" is a branding term NEB uses to describe reagents manufactured at NEB’s Rowley facility. The Rowley facility was designed to manufacture reagents under more rigorous infrastructure and process controls to achieve more stringent product specifications and customer requirements. Reagents manufactured at NEB’s Rowley facility are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards. However, at this time, NEB does not manufacture or sell products known as Active Pharmaceutical Ingredients (APIs), nor does NEB manufacture its products in compliance with all of the Current Good Manufacturing Practice regulations.

Catalog #	Concentration	Size
R0712S	10,000 units/ml	500 units
R0712L	10,000 units/ml	2,500 units
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Product Information

Product Source

An E. coli strain that carries the cloned BspQI gene from Bacillus sphaericus (X.S. Pan)

Reagents Supplied

The following reagents are supplied with this product:

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NEB # Component Name Component # Stored at (°C) Amount Concentration

R0712S -20

BspQI R0712SVIAL -20 1 x 0.05 ml 10,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

R0712L -20

BspQI R0712LVIAL -20 1 x 0.25 ml 10,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

This product is related to the following categories:: Restriction Endonucleases B,; Time-Saver Qualified Restriction Enzymes Products

This product can be used in the following applications:: Golden Gate Assembly,; Fast Cloning: Accelerate your cloning workflows with reagents from NEB,; Restriction Enzyme Digestion

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 50°C in a total reaction volume of 50 µl.
Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 50°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl₂
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 100%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent B
Storage Buffer

20 mM Tris-HCl
500 mM KCl
1 mM DTT
0.1 mM EDTA
500 µg/ml BSA
50% Glycerol
0.1% Triton® X-100
pH 7 @ 25°C

Heat Inactivation
80°C for 20 min
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Isoschizomers

LguI
PciSI
SapI

Activity at Temperature
@37°C: 50%
Related Products
Companion Products
Product Notes
1. Based on the stability of the enzyme in the reaction, incubations longer than 1 hr will not result in improved digestion, unless additional enzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
2. Star activity may result in a low salt buffer, such as rCutSmart Buffer. For an optimal restriction digest protocol please see NEBCloner.
3. Not sensitive to CpG, dcm, or dam methylation.
4. star activity may result from a glycerol concentration of >5%