BspQI

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. Based on the stability of the enzyme in the reaction, incubations longer than 1 hr will not result in improved digestion, unless additional enzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
2. Star activity may result in a low salt buffer, such as rCutSmart Buffer. For an optimal restriction digest protocol please see NEBCloner.
3. Not sensitive to CpG, dcm, or dam methylation.
4. star activity may result from a glycerol concentration of >5%

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Heat Inactivation
• NEBuffer Activity/Performance Chart with Restriction Enzymes
• Optimizing Restriction Endonuclease Reactions
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Single Letter Codes
• Star Activity
• Traditional Cloning Quick Guide

• Alphabetized List of Recognition Specificities
• Cleavage of Supercoiled DNA
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Dam-Dcm and CpG Methylation
• Enzymes with Nonpalindromic Sequences
• Frequencies of Restriction Sites
• Isoelectric Points (pI) for Restriction Enzymes
• Isoschizomers
• NEB Diluent and Buffer Table
• Time-Saver™ Qualified Enzymes
• Type IIS Restriction Enzymes
• Why Choose Recombinant Enzymes?

• Competitor Cross-Reference Tool
• DNA Sequences and Maps Tool

• Double Digest Finder
• Enzyme Finder
• NEBcutter™ v2.0
• NEBioCalculator®
• REBASE®

1. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
2. Why is my Restriction Enzyme not cutting DNA?
3. Why do I see a DNA smear on an agarose gel after a restriction digest?
4. Why do I see additional DNA bands on my gel after a restriction digest?
5. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
6. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
7. Does NEB offer any BSA-free and/or animal origin-free restriction enzymes for linearization of plasmids for mRNA vaccine development?
8. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

• Restriction Enzyme Troubleshooting Guide

• Golden Gate Assembly brochure

Product Citation Tool

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0712S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0712S_L_v1_0021301
• R0712S_L_v1_0021303
• R0712S_L_v1_0021308
• R0712S_L_v1_0021401
• R0712S_L_v1_0021404
• R0712S_L_v1_0021407
• R0712S_L_v1_0021411
• R0712S_L_v1_0021501
• R0712S_L_v1_0021504
• R0712S_L_v1_0021409
• R0712S_L_v1_0021507
• R0712S_L_v1_0021509
• R0712S_L_v1_0021511
• R0712S_L_v2_0021602
• R0712S_L_v2_0021606
• R0712S_L_v1_0021602
• R0712S_L_v1_0021606
• R0712S_L_v2_0021701
• R0712S_L_v2_0021706
• R0712S_L_v2_0021712
• R0712S_v2_10014986
• R0712L_v2_10014984
• R0712S_v2_10025868
• R0712L_v2_10025864
• R0712L_v2_10037231
• R0712S_v2_10037233
• R0712L_v2_10052832
• R0712S_v2_10058174
• R0712S_v2_10064760
• R0712L_v2_10069701
• R0712S_v2_10076496
• R0712S_v2_10080038
• R0712L_v2_10086321
• R0712S_v2_10091199
• R0712S_v2_10093546
• R0712L_v2_10097373
• R0712L_v2_10100070
• R0712L_v2_10106387
• R0712S_v2_10108647
• R0712L_v2_10109213
• R0712S_v2_10111561
• R0712L_v1_10117176

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• BspQI
• NEBuffer™ r3.1

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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