100% activity in CutSmart® Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
Standard Protocol for Restriction Enzyme Digests
NEB TV Ep. 15 – Applications of Restriction Enzymes
CutSmart Restriction Enzyme Buffer
Restriction Enzyme Digest Protocol: Cutting Close to DNA End
Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
Why is My Restriction Enzyme Not Cutting DNA?
Restriction Enzyme Digest Problem: Too Many DNA Bands
Double Digestion with NEBcloner
AleI-v2 is an engineered and improved version of AleI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)
Product SourceAn E. coli strain that carries the cloned AleI gene from Aureobacterium liquefaciens (S.K. Degtyarev).
The following reagents are supplied with this product:
Store at (°C) Concentration CutSmart® Buffer -20 10 X
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