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MfeI

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence C^AATT_G

Isoschizomers | Single Letter Code | Pronunciation:

Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.

100% activity in rCutSmart^™ Buffer (over 215 enzymes are available in the same buffer) allowing for easier double digests
High Fidelity (HF^®) version available (NEB #R3589) supplied with rCutSmart Buffer
Cut Site: C/AATTG

Catalog #	Concentration	Size
R0589S	Not Applicable	500 units
R0589L	Not Applicable	2,500 units
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Product Information

MfeI has a High Fidelity version, MfeI-HF® (NEB #R3589)

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Product Source

An E. coli strain that carries the MfeI gene from Mycoplasma fermentans (N.F. Halden).

Reagents Supplied

The following reagents are supplied with this product:

Show all

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0589S -20

MfeI R0589SVIAL -20 1 x 0.05 ml 10,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

R0589L -20

MfeI R0589LVIAL -20 1 x 0.25 ml 10,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Product Categories:: Restriction Endonucleases H M

Applications:: Restriction Enzyme Digestion

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium acetate
20 mM Tris-acetate
10 mM Magnesium acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 75%
NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 10%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent A
Storage Buffer

10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
No
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Isoschizomers

MfeI-HF
MunI
Related Products
Companion Products
Materials Sold Separately
- rCutSmart™ Buffer
Product Notes
1. MfeI is an isoschizomer of MunI.
2. Not sensitive to CpG, dcm, or dam methylation.
3. star activity may result from extended digestion.