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Home Restriction Endonucleases Products MfeI

MfeI

Mfe-I-cutsite_1

Isoschizomers | Single Letter Code | Pronunciation:

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.

100% activity in CutSmart^® Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
High Fidelity (HF^®) version available (NEB #R3589) supplied with CutSmart Buffer

Learn more about how this product is being used in the Product Citation Tool

Catalog #	Concentration	Size
R0589S	10,000 units/ml	500 units
R0589L	10,000 units/ml	2,500 units
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Product Information
MfeI has a High Fidelity version, MfeI-HF® (NEB #R3589)

High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Product Source

An E. coli strain that carries the MfeI gene from Mycoplasma fermentans (N.F. Halden).

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration

CutSmart® Buffer -20 10 X

Product Categories:

Restriction Endonucleases H M

Applications:

Restriction Enzyme Digestion
- Properties & Usage
  Unit Definition
  One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
  Reaction Conditions
  
  1X CutSmart® Buffer
  Incubate at 37°C
  
  1X CutSmart® Buffer
  50 mM Potassium Acetate
  20 mM Tris-acetate
  10 mM Magnesium Acetate
  100 µg/ml BSA
  (pH 7.9 @ 25°C)
  
  Activity in NEBuffers
  CutSmart® Buffer: 100%
  NEBuffer™ 1.1: 75%
  NEBuffer™ 2.1: 50%
  NEBuffer™ 3.1: 10%
  
  Diluent Compatibility
  
  Diluent A
  
  Storage Temperature
  -20°C
  Storage Conditions
  
  10 mM Tris-HCl
  50 mM NaCl
  1 mM DTT
  0.1 mM EDTA
  200 µg/ml BSA
  50% Glycerol
  pH 7.4 @ 25°C
  
  Heat Inactivation
  No
  Methylation Sensitivity
  
  dam methylation: Not Sensitive
  dcm methylation: Not Sensitive
  CpG Methylation: Not Sensitive
- Related Products
  Companion Products
  
  Nuclease-free Water
  
  MfeI-HF®
  
  Monarch® Plasmid Miniprep Kit
  
  Monarch® DNA Gel Extraction Kit
  
  Monarch® PCR & DNA Cleanup Kit (5 μg)
  
  Materials Sold Separately
  
  CutSmart® Buffer
- Product Notes
  MfeI is an isoschizomer of MunI.
  
  Not sensitive to CpG, dcm, or dam methylation.
  
  Star activity may result from extended digestion.
Protocols, Manuals & Usage
- Protocols
  Optimizing Restriction Endonuclease Reactions
  
  Double Digest Protocol with Standard Restriction Enzymes
  
  Restriction Digest Protocol
- Usage Guidelines
  Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
  
  Activity of Restriction Enzymes in PCR Buffers
  
  Alteration of Apparent Recognition Specificities Using Methylases
  
  Cleavage Close to the End of DNA Fragments
  
  Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
  
  Double Digests
  
  Heat Inactivation
  
  Reduced Star Activities of HF® Enzymes
  
  Restriction Endonucleases - Survival in a Reaction
  
  Restriction Enzyme Diluent Buffer Compatibility
  
  Restriction Enzyme Tips
  
  Single Letter Codes
  
  Star Activity
Tools & Resources
- Selection Charts
  Alphabetized List of Recognition Specificities
  
  Buffer and Diluent Formulation Table
  
  Compatible Cohesive Ends and Generation of New Restriction Sites
  
  Cross Index of Recognition Sequences
  
  Dam-Dcm and CpG Methylation
  
  Frequencies of Restriction Sites
  
  Isoschizomers
  
  Recleavable Filled-in 5' Overhangs
  
  Time-Saver™ Qualified Enzymes
- Web Tools
  DNA Sequences and Maps Tool
  
  Double Digest Finder
  
  Enzyme Finder
  
  NEBcutter V2.0
  
  NEBioCalculator
  
  REBASE
FAQs & Troubleshooting
- FAQs
  Is MfeI inhibited by salt?
  
  Does MfeI have trouble cleavin PCR products?
  
  What is the difference between MfeI and MunI, the isoschizomer it replaced.
  
  What is the molecular weight of MfeI?
  
  Does MfeI have single sites in common vectors?
  
  What is the activity of MfeI at 25°C?
  
  My enzyme is no longer Time-Saver™ qualified. What happened?
  
  I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  
  Why is my Restriction Enzyme not cutting DNA?
  
  Why do I see a DNA smear on an agarose gel after a restriction digest?
  
  Why do I see additional DNA bands on my gel after a restriction digest?
  
  How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
  
  Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  R0589S_L_v1
  
  R0589S_L_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  R0589S_L_v1_0081402
  
  R0589S_L_v1_0081407
  
  R0589S_L_v1_0081404
  
  R0589S_L_v1_0081409
  
  R0589S_L_v1_0081411
  
  R0589S_L_v1_0071212
  
  R0589S_L_v1_0081305
  
  R0589S_L v1_0081311
  
  R0589S_L_v1_0081503
  
  R0589S_L_v1_0081509
  
  R0589S_L_v1_0081510
  
  R0589S_L_v1_0081602
  
  R0589S_L_v1_0081604
  
  R0589S_L_v1_0081607
  
  R0589S_L_v2_0081611
  
  R0589S_L_v2_0081703
  
  R0589S_L_v2_0081705
  
  R0589S_L_v2_0081710
  
  R0589S_L_v2_0081712
  
  R0589S_L_v2_0081803
  
  R0589L_v2_10012877
  
  R0589S_v2_10012879
  
  R0589L_v2_10021707
  
  R0589L_v2_10028201
  
  R0589S_v2_10028199
  
  R0589L_v2_10034798
  
  R0589S_v2_10034800
  
  R0589L_v2_10043669
  
  R0589S_v2_10043671
  
  R0589L_v2_10052857
  
  R0589S_v2_10052860
  
  R0589L_v2_10064278
  
  R0589S_v2_10064280
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  MfeI
  
  CutSmart® Buffer
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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