cloned at NEB recombinant neb31 dil_A 37 No

This product has been replaced with the high fidelity version, MfeI-HF™ (NEB #R3589).

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at

  • Catalog # R0589 was discontinued on December 15, 2021
NEB restriction endonuclease that recognizes the sequence C^AATT_G
  • 100% activity in rCutSmart Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • High Fidelity (HF®) version available (NEB #R3589) supplied with rCutSmart Buffer
  • Restriction Enzyme Cut Site: C/AATTG
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