cloned at NEB recombinant neb31 2site dil_A 37 65 Heat CpG dam

SAM now included in enzyme formulation – no longer supplied as a separate vial

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at

NEB restriction endonuclease that recognizes the sequence _NN^NNNNNNNNNNCGANNNNNNTGCNNNNNNNNNN_NN^
  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.

  • Restriction Enzyme Cut Site: (10/12)CGANNNNNNTGC(12/10)

Catalog # Concentration Size
R0545S 2,000 units/ml 250 units
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