Non-T7 Expression

The NEBExpress strain is a multiple purpose expression strain capable of robust expression from lac, tac, trc, arabinose-inducible and rhamnose-inducible promoters. NEBExpress Competent E. coli (High Efficiency) (NEB #C2523) are chemically competent E. coli cells suitable for high efficiency transformation and protein expression. They are the recommended host strain for the NEBExpress MBP Fusion and Purification System (NEB #E8201). NEBExpress E.coli does not restrict methylated DNA (McrA-, McrBC-, EcoBr-m-, Mrr-). NEBExpress Iq Competent E. coli (NEB #C3037) and BL21 Competent E. coli (NEB #C2530) strains may also be used with pMAL vectors or other vectors with a tac, trclac, or araBAD promoter.

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FAQs for Non-T7 Expression
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