Taq DNA Polymerase is the industry standard for routine PCR. Taq with Standard Taq Buffer (NEB #M0273) is available in economical extra-large pack sizes. NEB provides high quality recombinant Taq at an exceptional value. Taq is available with different formats to accommodate a variety of PCR applications. For example, Taq with standard Taq Buffer is designed to support existing PCR platforms, while Hot Start Taq formulations (NEB #M0495) are also available for flexibility during reaction setup and increased specificity.
Taq DNA Polymerase is available in several convenient formats. The Taq PCR kit (NEB #E5000) includes all the reagents for 200 reactions. Taq 2X (NEB #M0270) or 5X (NEB #M0285) Master Mixes are ideal for high-throughput applications. Quick-Load® Taq 2X Master Mix (NEB #M0271) includes dye for direct gel loading.For exceptional performance across a wide range of templates, try OneTaq or OneTaq Hot Start.
ThermoPol™ is a trademark of New England Biolabs, Inc.
Quick-Load® is a register trademark of New England Biolabs, Inc.
- PCR Protocol for Crimson Taq DNA Polymerase (M0324)
- PCR Protocol for Crimson Taq DNA Polymerase with (Mg-free) Buffer (M0325)
- Protocol for OneTaq 2X Master Mix with GC Buffer (M0483)
- Protocol for OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (M0488)
- OneTaq® Quick-Load® 2X Master Mix with GC Buffer (M0487)
- Protocol for a Routine Taq PCR
- Taq DNA Polymerase Guidelines for PCR Optimization
- PCR Protocol for Taq DNA Polymerase
- PCR Protocol for Taq DNA Polymerase with Standard Taq (Mg-free) Buffer (M0320)
- PCR Protocol for Taq DNA Polymerase with ThermoPol Buffer (M0267)
- PCR Protocol for Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer (M0321)
- Protocol for OneTaq Hot Start DNA Polymerase (M0481)
- Protocol for OneTaq® 2X Master Mix with Standard Buffer (M0482)
- Protocol for OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer
- Protocol for OneTaq Hot Start 2X Master Mix with GC Buffer (M0485)
- Protocol for OneTaq Hot Start 2X Master Mix with Standard Buffer (M0484)
- Protocol for a Routine PCR with Phusion® High-Fidelity PCR Kit
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Protocol for OneTaq® Quick-Load 2X Master Mix with Standard Buffer (M0486)
- Multiplex PCR Guidelines for Multiplex PCR 5X Master Mix
- Protocol for Quick-Load® Taq 2X Master Mix
- Protocol for Taq 2X Master Mix (M0270)
- Protocol for Taq 5X Master Mix (M0285)
- Quick Protocol for Multiplex PCR 5X Master Mix
- EpiMark® Hot Start Taq DNA Polymerase Guidelines for PCR (M0490)
- Protocol for a PCR reaction using Hot Start Taq 2X Master Mix (M0496)
- PCR Using Hot Start Taq DNA Polymerase (M0495)
- PCR Protocol for OneTaq® DNA Polymerase (M0480)
- A-Tailing with Taq Polymerase
Understanding Variability in DNA Amplification Reactions
Anatomy of a Polymerase - How Function and Structure are Related
Read about the relationship between Polymerase structure and function when copying DNA.
- PCR Brochure
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- Taq PCR Kit Troubleshooting Guide
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
- Vladimir Potapov, Jennifer L. Ong. (2017) Examining Sources of Error in PCR by Single-Molecule Sequencing. PLOS One; PubMedID: 28683110
Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template?
Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end?
Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable?
Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?
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This overview will walk you through how the Polymerase Chain Reaction (PCR) works.