DNA polymerases with high fidelity are important for applications in which the DNA sequence needs to be correct after amplification. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Reagent selection includes a standalone enzyme, master mix and kit format, as well as a choice of reaction buffers for amplification of difficult templates. Phusion Hot Start Flex DNA Polymerase is available as standalone enzyme or in a master mix format and enables high specificity amplification of a broad range of templates. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons.
Thermo Scientific® is a registered trademark of Thermo Fisher Scientific.
Phusion® was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.
- PCR Protocol Phusion® DNA Polymerase
- Protocol for Phusion® Hot Start Flex 2X Master Mix
- PCR Protocol for Phusion® Hot Start Flex DNA Polymerase (M0535)
- Protocol Phusion® High-Fidelity PCR Master Mix with HF Buffer
- PCR Optimization with Phusion® High-Fidelity PCR Kit
- Protocol for a Routine PCR with Phusion® High-Fidelity PCR Kit
- PCR Optimization of the Control Template using Phusion® High-Fidelity PCR Kit
- Protocol for Phusion® High-Fidelity PCR Master Mix with GC Buffer
Understanding Variability in DNA Amplification Reactions
Anatomy of a Polymerase - How Function and Structure are Related
Read about the relationship between Polymerase structure and function when copying DNA.
- PCR Brochure
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template?
Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end?
Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable?
Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.