Deep Vent® (exo-) DNA Polymerase
Deep Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the 3′→ 5′ proofreading exonuclease activity associated with Deep Vent DNA Polymerase. Deep Vent (exo-) DNA Polymerase is even more stable than Vent® (exo-) DNA Polymerase with a half-life of 23 hours at 95°C and 8 hours at 100°C. Both Deep Vent (exo-) and Vent (exo-) DNA Polymerase are suitable for primer extensions and high temperature (72°C) DNA sequencing.
Deep Vent® DNA Polymerase
Deep Vent DNA Polymerase is the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3′→ 5′ proofreading exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100°C.
Vent® (exo-) DNA Polymerase
Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the 3′→ 5′ proofreading exonuclease activity associated with Vent DNA Polymerase (1). This is the preferred form for high-temperature dideoxy sequencing reactions and for high yield primer extension reactions. The fidelity of polymerization by this form is reduced to a level about 2-fold higher than that of Taq DNA Polymerase (2,3).
Vent® DNA Polymerase
Vent DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA Polymerase is 5-15-fold higher than that observed for Taq DNA Polymerase (1,2). This high fidelity derives in part from an integral 3′→5′ proofreading exonuclease activity in Vent DNA Polymerase (1,3). Greater than 90% of the polymerase activity remains following a 1 hour incubation at 95°C.
Therminator™ DNA Polymerase
Therminator DNA Polymerase is a 9°N™ DNA Polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides (1,2).
- Protocol for a Routine Deep Vent® PCR
- Guidelines for PCR Optimization for Deep Vent® DNA Polymerase
- Guidelines for PCR Optimization for Vent DNA Polymerase
- PCR Protocol for Hemo KlenTaq®
- Protocol for a Routine Deep Vent® (exo-) PCR
- Protocol for a Routine Vent PCR
- Protocol for a Routine Vent (exo-) PCR
- Vladimir Potapov, Jennifer L. Ong. (2017) Examining Sources of Error in PCR by Single-Molecule Sequencing. PLOS One; PubMedID: 28683110
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