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Nucleotide Solutions

New England Biolabs supplies a selection of highly pure nucleotides for use with DNA and RNA polymerases.

Deoxynucleotide Solution Set (NEB #N0446) 

The Deoxynucleotide Solution Set contains four separate 100 mM solutions of ultrapure nucleotides (dATP, dCTP, dGTP, and dTTP).

Deoxynucleotide Solution Mix (NEB #N0447)

The Deoxynucleotide Solution Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP. Each nucleotide is present at a concentration of 10 mM in the mixture for a total dNTP concentration of 40 mM.

7-deaza-dGTP* (NEB #N0445)

7-deaza-dGTP is a useful additive for the PCR of GC-rich templates; contains a 5 mM solution of 7-deaza-dGTP as a dilithium salt.
* licensed from Roche Diagnostics GmbH

Acyclonucleotide Set (NEB #N0460)

The Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP), supplied as a dry powder.

dATP Solution (NEB #N0440)

The dATP solution contains 0.25 ml of 100 mM ultrapure dATP.

Ribonucleotide Solution Set (NEB #N0450)

Ribonucleotide Solution Set consists of four separate 100 mM solutions of ATP, GTP, CTP and UTP.

Ribonucleotide Solution Mix (NEB #N0466)

The Ribonucleotide Solution Mix is an equimolar mixture of ribonucleotide triphosphates (rATP, rCTP, rGTP and rUTP). Each is supplied at a concentration of 80 mM for a total concentration of 320 mM.

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FAQs for Nucleotide Solutions

Protocols for Nucleotide Solutions

DNA Polymerase Selection Chart

NEB offers a guidelines for choosing the correct DNA polymerase for your application by providing a list of specific properites.
Several factors govern which polymerase should be used in a given application, including: 

Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template? 

Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end? 

Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable? 

Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?

PCR Selection Tool

Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.

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