SNAP-tag® Purified Protein

The SNAP-tag® is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT).

  • The purified SNAP-tag protein is supplied at 1 mg/ml (50 μM) concentration in 1X PBS solution containing 1 mM DTT at 1 mg/ml (50 μM)
  • Mass spectrometry analysis indicates the SNAP-tag purified protein has two species with molecular weights of 19,694 and 19,829 (with a C-terminal DTT moiety attached)
  • SNAP-tag Purified Protein can be used as a positive control for in vitro labeling with various SNAP-tag fluorescent substrates
Catalog # Concentration Size
P9312S 50 µM 50 µg
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  • Product Information
    The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    SNAP-tag Purified Protein can be used as a positive control for in vitro labeling with various SNAP-tag fluorescent substrates. The coding sequence of SNAP-tag was cloned into a pTXB1 derived E. coli T7 expression vector. SNAP-tag protein (MW: 19,694) was expressed and purified according to the instructions in the IMPACT™ kit manual (NEB #E6901). The purified SNAP-tag protein was dialyzed into 1X phosphate buffered saline (PBS) solution containing 1 mM DTT at 1 mg/ml (50 μM) and stored at -80°C.

    Mass spectrometry analysis indicates the SNAP-tag purified protein has two species with molecular weights of 19,694 and 19,829 (with a C-terminal DTT moiety attached).

    P9312_thumb
    Product Categories:
    Snap Tag Other,
    Protein Labeling Products
    Applications:
    SNAP Companion,
    Protein Labeling Snap Clip,
    Cell Imaging
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