Factor Xa Protease
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.
- Compatible for use with the pMAL™ Protein Fusion and Purification System (NEB #E8200)
- Allows for convenient removal of MBP by loading the MBP-fusion digest onto amylose resin and collecting your protein of interest in the flow through
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate (1,2,3). The most common secondary site, among those that have been sequenced, is Gly-Arg. There seems to be a correlation between proteins that are unstable in E.coli and those that are cleaved by Factor Xa at secondary sites; this may indicate that these proteins are in a partially unfolded state (Walker, I., Riggs, P., unpublished observations). Factor Xa will not cleave a site followed by proline or arginine.
Product SourceFactor Xa Protease is purified from bovine plasma and activated by treatment with the activating enzyme from Russell's viper venom.
- Product Categories:
- Proteases Products
- Protein Digestion
- Protocols, Manuals & Usage
- Tools & Resources
- Citations & Technical Literature
- Quality, Safety & Legal
Other Products You May Be Interested In
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.