Color Prestained Protein Standard, Broad Range (10-250 kDa)

Features

• Direct loading, additional loading buffer and heat incubation not required
• Applications include verification of western blot transfer efficiency on membranes and fluorescent imaging of SDS-PAGE

Companion Products

• Unstained Protein Standard, Broad Range (10-200 kDa)
• Blue Prestained Protein Standard, Broad Range (11-250 kDa)

1. To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.
2. To maximize shelf life, store at -20°C. Guaranteed stable for 2 years when properly stored.
3. It is not necessary to pre-heat the unstained protein standard prior to loading on a gel.
4. Recommended mini-gel load: 3 μl.
5. Apparent molecular weight values for prestained proteins can be different when run on different gel types and percentages.

1. Laemmli, UK (1970). Nature. 227, 680.
2. Sambrook, J., Fitsch, EF. and Maniatis, T. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

1. Protocol for Using the Color Prestained Protein Standard, Broad Range (10-250 kDa) (NEB #P7719) for an SDS-PAGE Mini Gel

1. How does the new Color Prestained Protein Standard, Broad Range differ from NEB’s previous Color Prestained Protein Standard?
2. What is the protein concentration of the proteins used in the Color Prestained Protein Standard, Broad Range?
3. I accidentally left my Protein Standard out at room temperature for a number of days. Is it still okay to use?
4. I notice that the molecular weights I am observing are slightly different than those reported. Can you explain?
5. What volume is supplied in a small pack and a large pack of NEB’s Protein Standards?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P7719S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P7719G_v0_10040353
• P7719L_v1_10040352
• P7719S_v1_10040351
• P7719G_v0_10043934
• P7719L_v1_10049242
• P7719S_v1_10049243
• P7719G_v1_10052748
• P7719S_v1_10056021
• P7719S_v1_10058286

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Color Prestained Protein Standard, Broad Range (10-250 kDa)

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.