Home Discontinued Products Blue Prestained Protein Standard, Broad Range (11-190 kDa)

Blue Prestained Protein Standard, Broad Range (11-190 kDa)

This product has been discontinued and replaced with NEB #P7718. There may be some remaining stock in certain geographies; please contact your local subsidiary office to inquire.

Catalog # P7706 was discontinued on May 01, 2019

The Blue Prestained Protein Standard, Broad Range is a mixture of 11 highly-purified, prestained proteins, covalently coupled with a blue chromophore, that resolves into 11 sharp bands when electrophoresed.

Allows approximate molecular weight determination when performing SDS-PAGE analysis.
Applications include verification of Western transfer efficiency on membranes and fluorescent imaging of SDS-PAGE
Direct loading, additional loading buffer and heat incubation not required
No detectable protease-contaminating activities
Optimal stability for up to 24 months

Product Information
Blue Prestained Protein Standard, Broad Range is a mixture of highly purified, prestained proteins, covalently coupled with a blue chromophore, that resolves into 11 sharp bands when electrophoresed. The Blue Protein Standard, Broad Range is designed for observing protein separation during SDS-PAGE, verification of western transfer efficiency on membranes and for approximating the size of proteins (1–3). Blue Prestained Protein Standard, Broad Range may also be used in fluorescent imaging of SDS-PAGE.

This standard is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agents before loading.

Product Categories:

Discontinued Products
- Properties & Usage
  
  Storage Temperature
  -20°C
  Storage Conditions
  
  20 mM Tris-phosphate
  2% SDS
  0.2 mM DTT
  3.6 M Urea
  15% Glycerol
  pH 7.5 @ 25°C
- Product Notes
  To maximize shelf life, store at -20°C. Guaranteed stable for 2 years when properly stored.
  
  Apparent molecular weight values for prestained proteins can bedifferent when run on different gel types and percentages. Severalcommonly-used gels were tested and the results can be found in theFrequently Asked Questions (FAQ) section of the product page.
  
  It is important to note that the Blue Protein Standard, Broad Range does not have a dye front indicating that the gel is near completion. Please monitor the gel carefully to ensure that the lower molecular weight bands are not accidentally run off the gel.
- References
  Ma, D. and Xu, MQ. New England Biolabs, Inc. Unpublished observation
  
  Laemmli, UK. (1970). Nature. 227, 680.
  
  Sambrook, J., Fitsch, EF. and Maniatis, T. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
Protocols, Manuals & Usage
- Protocols
  Protocol for Using the Blue Prestained Protein Standard, Broad Range (P7706) for an SDS-PAGE Mini Gel
FAQs & Troubleshooting
- FAQs
  What is the protein concentration of the proteins used in the Prestained Standard?
  
  I notice that the molecular weights I am observing are slightly different than those reported. Can you explain?
  
  What volume of Blue Prestained Protein Standard, Broad Range (11-190 kDa) is supplied in a small pack (P7706S) and a large pack (P7706L)?
  
  I accidentally left my Protein Standard out at room temperature for a number of days. Is it still okay to use?
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  P7706S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  P7706S_L_v1_0101711
  
  P7706L_v1_10007549
  
  P7706S_v1_10007550
  
  P7706S_v1_10018077
  
  P7706L_v1_10018076
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Blue Prestained Protein Standard, Broad Range (11-190 kDa)
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.