Bacteroides Heparinase III (also called Heparin Lyase III) is cloned from Bacteroides eggerthii. It is active on both heparin and heparan sulfate.
Bacteroides Heparinase III can cleave the glycosidic bond between hexosamines and either iduronic acid or glucuronic acid residues. It is active in the presence of 6-sulfation. The reaction yields oligosaccharide products containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm
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