PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F (Glycerol-free), Recombinant is a recombinant amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
Peptide: N-Glycosidase F, also known as PNGase F, is a recombinant amidase which is supplied glycerol free for optimal performance in HPLC intensive methods. PNGase F cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins (1).
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Glycoprotein Denaturing Buffer||-20||10 X|
|GlycoBuffer 2||-20||10 X|
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