Microbiome DNA Enrichment

Microbiome DNA analysis can be challenging due to the high percentage of host DNA present in many samples. The NEBNext® Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment. The NEBNext Microbiome DNA Enrichment Kit is suitable for a wide range of sample types and is compatible with downstream applications including next generation sequencing on all platforms, qPCR and end-point PCR.
nebnext selector inline banner

Choose Type:

FAQs for Microbiome DNA Enrichment
Protocols for Microbiome DNA Enrichment
    Publications related to Microbiome DNA Enrichment
    • George R. Feehery, Erbay Yigit, Samuel O. Oyola, Bradley W. Langhorst, Victor T. Schmidt, Fiona J. Stewart, Eileen T. Dimalanta, Linda A. Amaral-Zettler, Theodore Davis, Michael A. Quail, Sriharsa Pradhan (2013) A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA PLoS One; 8 (10), e76096 . PubMedID: 24204593
    • Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey (2014) Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. Appl Plant Sci; 2 (11), 1400064. PubMedID: 25383266, DOI: 10.3732/apps.1400064
Posters and Manuals
Microbiome DNA Enrichment Workflow

workflow

Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos