Microbiome DNA Enrichment
Choose Type:
- Agencourt® Ampure® Beads (Beckman Coulter) Preparation - NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Adaptor Ligation (E6090)
- NEBNext dA-Tailing Module Protocol (E6053)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- NEBNext End Repair Module Protocol (E6050)
- NEBNext DNA Sample Prep Master Mix Set for 454 End Repair Module Protocol (E6070)
- NEBNext DNA Sample Prep Master Mix Set for 454 Fill-in and ssDNA Isolation Module Protocol (E6070, E6071)
- NEBNext Quick Ligation Module Protocol (E6056)
- NEBNext DNA Sample Prep Master Mix Set for 454 Quick Ligation Module Protocol (E6070)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Small Fragment Removal (E6090)
- Protocol for use with NEBNext Microbiome DNA Enrichment (E2612)
- Protocol for use with NEBNext DNA Library Prep Master Mix Set for Illumina (E6040)
- Setting up the PCR Reaction - NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (E7600)
- Refer to the manual (NEB #E7335) for a list of NEBNext library prep kits that contain protocols for use of NEBNext adaptors and primers.
- Refer to the manual for a list of NEBNext library prep kits that contain protocols for use of NEBNext adaptors and primers.
- Data Analysis - NEBNext Library Quant Kit (E7630)
- Experimental Considerations - NEBNext Library Quant Kit (E7630)
- NEBNext Library Quant Kit Protocol - NEBNext Library Quant Kit (E7630)
- NEBNext Library Quant Kit Quick Protocol (E7630)
- Expected Results - NEBNext Library Quant Kit (E7630)
- Protocol for use with NEBNext® Ultra™ II End Repair/dA-Tailing Module (E7546)
- Protocol for a PCR reaction using NEBNext® Ultra™ II Q5® Master Mix (M0544)
- Setting up the PCR Reactions < 96 Samples and 96 Samples - 96 Single Index Kit (E6609)
- Index Pooling Guidelines - 96 Single Index Kit (E6609)
- Setting up the PCR Reaction - NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) (E7780)
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Addressing Challenges in Microbiome DNA Analysis
Microbiome sample analysis is commonly confounded by the presence of host-cell DNA in the sample. The NEBNext® Microbiome DNA Enrichment Kit enables enrichment of non-CpG-methylated DNA from samples contaminated with eukaryotic-cell DNA.
- NEBNext for Ion Torrent™ Brochure
Feature Articles
Brochures
- George R. Feehery, Erbay Yigit, Samuel O. Oyola, Bradley W. Langhorst, Victor T. Schmidt, Fiona J. Stewart, Eileen T. Dimalanta, Linda A. Amaral-Zettler, Theodore Davis, Michael A. Quail, Sriharsa Pradhan 2013. A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA PLOS ONE. 8 (10), PubMedID: 24204593, DOI:
- Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey 2014. Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. Applications in Plant Sciences. 2 (11), PubMedID: 25383266, DOI: 10.3732/apps.1400064
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Videos
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Behind the Paper: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues
Can microbiome-enrichment techniques be used to partition plant DNA? Erbay describes the findings of his recent paper.
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NEBNext Ultra II DNA Library Prep Protocol
This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.
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Library prep for NGS
Get a quick overview of NGS library preparation from an NEB scientist.
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Tips for Optimizing DNA Inputs in NGS Library Construction
Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips for DNA quantification and assessing purity.
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12 Quick Tips for NGS Library Preparation
These 12 quick tips for NGS library preparation are a great refresher if you're a seasoned pro, or a great introduction if you're a beginner.
