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DNA Library Prep

NEBNext® reagents are available for sample preparation of genomic DNA, ChIP DNA, and FFPE DNA. These products are provided in user-friendly formats including kits and modules, with bulk and customized formats also available. Adaptors and primers are available separately, for maximum flexibility. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

NEBNext Ultra™ II FS (E7805S/L, E6177S/L) provides a fast and reliable solution for DNA fragmentation and library construction. A new enzymatic DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content. For mechanically-sheared DNA, NEBNext Ultra™ II (E7645S/L, E7103S/L) enables the construction of high yield, high quality libraries from low picogram to microgram input amounts.

For methylome analysis, NEBNext Enzymatic Methyl-seq (E7120S/L) provides a high-performance enzyme-based alternative to bisulfite conversion. The consistently high conversion performance and minimized DNA damage with the EM-seq™ protocol, in combination with highly efficient Ultra II library prep, result in superior detection of CpGs with fewer sequencing reads.

NEBNext Oligos are optimized to maximize library yields and validated for specific applications. A large number of highly pure index primers are available, in unique dual, dual and single index primers formats.  NEBNext Oligos support index insertion during library amplification.  The NEBNext Adaptor’s hairpin-loop structure ligates at high efficiency and minimizes adaptor-dimer formation. For EM-seq™ and bisulfite workflows, a modified adaptor is available. 

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FAQs for DNA Library Prep
Protocols for DNA Library Prep
Application Notes for DNA Library Prep
Posters and Manuals
Ultra II DNA Library Preparation Workflow for Illumina
Ultra DNA Library Preparation Workflow for Illumina
DNA Library Prep Workflow for Ion Torrent
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.