Microbiome DNA analysis can be challenging due to the high percentage of host (eukaryotic) DNA present in many samples. The NEBNext® Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment. The NEBNext Microbiome DNA Enrichment Kit is suitable for a wide range of sample types and is compatible with downstream applications including next generation sequencing on all platforms, qPCR and end-point PCR.
The Kit is also effective for separation of organelle DNA (e.g. mitochondria, chloroplast) from eukaryote nuclear DNA.
For a closer look at the NEBNext Microbiome DNA Enrichment Kit, read “Addressing Challenges in Microbiome DNA Analysis”.
Addressing Challenges in Microbiome DNA Analysis
Microbiome sample analysis is commonly confounded by the presence of host-cell DNA in the sample. The NEBNext® Microbiome DNA Enrichment Kit enables enrichment of non-CpG-methylated DNA from samples contaminated with eukaryotic-cell DNA.
- NEBNext for Ion Torrent™ Brochure
- Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey (2014) Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. Appl Plant Sci; 2 (11), 1400064. PubMedID: 25383266, DOI: 10.3732/apps.1400064
- George R. Feehery, Erbay Yigit, Samuel O. Oyola, Bradley W. Langhorst, Victor T. Schmidt, Fiona J. Stewart, Eileen T. Dimalanta, Linda A. Amaral-Zettler, Theodore Davis, Michael A. Quail, Sriharsa Pradhan (2013) A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA PLoS One; 8 (10), e76096 . PubMedID: 24204593
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Behind the Paper: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues
Can microbiome-enrichment techniques be used to partition plant DNA? Erbay describes the findings of his recent paper.
This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.
Get a quick overview of NGS library preparation from an NEB scientist.
Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips for DNA quantification and assessing purity.
These 12 quick tips for NGS library preparation are a great refresher if you're a seasoned pro, or a great introduction if you're a beginner.