|FEATURES||ULTRA II DNA LIBRARY PREP||ULTRA II FS DNA LIBRARY PREP|
|Unprecedented performance, enabling lower inputs and fewer PCR cycles||Perform fragmentation, end repair, and dA-tailing with a single enzyme mix|
|ULTRA II INPUT AMOUNTS||500 pg – 1 µg of sheared DNA||100 pg – 500 ng of intact DNA|
|Available with or without beads?|
|ULTRA II PCR-FREE INPUT AMOUNTS||250 ng – 1,000 ng of sheared DNA||50 ng – 500 ng of intact DNA|
|Available with or without beads?|
|COMPATIBLE WITH FFPE DNA?||Yes – for improved performance, consider the NEBNext FFPE DNA Repair Mix (NEB #M6630)||Yes – for improved performance, consider the NEBNext FFPE DNA Repair Mix (NEB #M6630)|
|COMPATIBLE WITH METHYLOME ANALYSIS?||Yes – Methylome analysis is supported; however, we recommend NEBNext EM-seq™ (NEB #E7120)||No – not compatible due to potential for loss of methyl marks|
|COMPATIBLE WITH OXFORD NANOPORE TECHNOLOGIES?||Yes. We recommend the NEBNext Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7810).|
NEBNext DNA library prep reagents are also available at scale for your high-throughput and/or development needs. Contact the Customized Solutions Team for more details on custom product options, large volumes, or OEM materials.
The Quantitation Question: How does accurate library quantitation influence sequencing?
The determination of the number of sequencing-ready molecules present after library preparation is an important step in the next generation sequencing (NGS) workflow and has a strong influence on the success of both a sequencing run and a sequencing-based experiment.
Improving Enzymatic DNA Fragmentation for Next Generation Sequencing Library Construction
Among the available options for DNA fragmentation, enzymatic fragmentation is demonstrably gentler on the sample, yielding less damage at any scale. The new NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® includes a single step for enzymatic fragmentation, end repair, and dA tailing.
- A Robust, Streamlined, Enzyme-based DNA Library Preparation Method Amenable to a Wide Range of DNA Inputs (2019)
- EM-seq™ Enables Accurate and Precise Methylome Analysis of Challenging DNA Samples (2019)
- NEBNext® Ultra™ II FS DNA: A Robust Enzyme-based DNA Library Preparation Method Compatible with Plant Samples
- Uncovering the Cannabis sativa Methylome Through Enzymatic Methyl-seq (2019)
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email email@example.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips for DNA quantification and assessing purity.
This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA
In this webinar, Laurence Etwiller and Jennifer Ong discuss their recent publications, and how library preparation affects sequencing accuracy and variant calling. View full Q & A summary.
Behind the paper: DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification
NEB researchers published a paper in Science highlighting DNA damage as a prevalent source of errors in public cancer databases. Learn about how addressing this damage could improve the detection of low-frequency disease variants.
This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.
Are you running a core sequencing facility? Watch as Eileen shares a few reasons that NEBNext products are particularly well suited to sequencing core labs.
These 12 quick tips for NGS library preparation are a great refresher if you're a seasoned pro, or a great introduction if you're a beginner.