There are two steps to using this system: subcloning and expression of the protein of interest as an ACP-tag fusion, and labeling of the fusion protein with the CoA substrate of choice. In this document, the cloning and expression of ACP-tag protein fusions is described. The labeling of fusion proteins with CoA substrates is described in the documentation supplied with CoA substrates and ACP or SFP Synthase.
DNASU is a central repository for plasmid clones and collections that may also be helpful.
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