There are two steps to using this system: sub cloning and expression of the protein of interest as a MCP-tag fusion, and labeling of the fusion protein using SFP Synthase with the substrate of choice. The cloning and expression of MCP-tag protein fusions is described in this document. The labeling of fusion proteins with CoA substrates is described in the documentation supplied with CoA substrates and SFP Synthase.
DNASU is a central repository for plasmid clones and collections that may also be helpful.
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