pMAL™-p5X Vector
The pMAL-p5X vector is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with Factor Xa protease (NEB #P8010). MBP fusions made with this vector include an N-terminal signal sequence, which directs the fusion protein to be localized in the periplasm.
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- The MBP has been engineered for tighter binding to amylose resin.
- Localization to periplasm can aid in protein folding and lead to higher yields of proteins containing disulfide bonds
- Ampicillin resistance
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The vector pMAL-p5X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa (NEB #P8010).
MBP fusions made with this vector include an N-terminal signal sequence, so the fusion protein is directed to the periplasm. The MBP has been engineered for tighter binding to amylose resin.
A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding maltose-binding protein). The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest.
VisitDNA Sequences and Maps page for more information.
DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.
- Product Categories:
- DNA Plasmids & Substrates Products,
- pMAL Products
- Applications:
- E Coli Protein Expression
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