pMAL™-p5X Vector

Companion Products

• NEB® 10-beta Competent E. coli (High Efficiency)
• NEBExpress^® Competent E. coli (High Efficiency)

1. NEB 10-beta E. coli (High Efficiency) (NEB #C3019) is recommended for propagation and subcloning. NEB Express Competent E.coli (High Efficiency) (NEB #C2523) is recommended for expression using this vector.
2. This vector conveys ampicillin resistance for propagation in E. coli.

1. Guan, C., Li, P., Riggs, P.D. and Inouye, H. (1987). Gene. 67, 21-30.
2. Maina, C.V., Riggs, P.D., Grandea, A.G.III, Slatko, B.E., Moran, L.S., Tagliamonte, J.A., McReynolds, L.A. and Guan, C. (1988). Gene. 74, 365-373.
3. Nagai, K. and Thogersen, H.C. (1987). Methods Enzymology. 153, 461-481.
4. Riggs, P.D. (1990). Expression and Purification of Maltose-Binding Protein Fusions. In F.M. Ausebel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl(Ed.), Methods Enzymology. 16.6.1-16.6.12. New York: John Wiley & Sons, Inc.
5. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985). Gene. 33, 103-119.

• DNA Sequences and Maps Tool

1. Where can I find many more detailed FAQs for pMAL Protein Fusion & Purification System (E8200)?
2. What strain(s) do you recommend as hosts for the pMAL vectors?
3. What are some of the possible explanations for an inability to clone an insert into a pMAL vector?
   
4. What are the differences between the generations of pMAL vectors (e.g. pMAL-c2 vs. pMAL-c5 vs. pMAL-c6 or pMAL-p2 vs. pMAL-p5)?
5. What is the DNA sequence of this product?
6. What primers should I use to sequence the ends of my insert after I clone it into a pMAL vector?

Product Citation Tool

• Over 40 years in protein expression and purification – a historical perspective

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• N8109S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N8109S_v1_10035056
• N8109S_v1_10051898
• N8109S_v1_10056657
• N8109S_v1_10061380

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• pMAL™-p5X Vector

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

 

The buyer/user has a non-exclusive license to use the vector for Research Purposes Only. Commercial use of this vector requires a license from New England Biolabs, Inc.

U.S. Patent No. 5,643,758