pMAL-pIII Vector

  • Catalog # N8101 was discontinued on January 05, 2015
  • Product Information
    The pMAL-pIII Vector is a derivative of pMAL-p2 in which the leader sequence of maltose binding protein (MBP, malE) has been replaced with the M13 pIII leader sequence. KpnI/Acc65I and EagI sites have been introduced within the pIII leader to facilitate direct transfer of sequences selected from any of the Ph.D. phage display peptide libraries into an expression vector. The corresponding peptides are expressed as N-terminal MBP fusions which bear some similarity to M13 pIII displayed peptides.These fusions can then be easily purified from E. coli periplasmic space by osmotic shock followed by affinity chromatography on amylose resin. Since the peptide is expressed at the N-terminus of MBP, Factor Xa cannot be used to cleave the peptide from MBP.
    pMAL-pIII cloning region. Note PhD fragments are inserted into the M13KE pIII leader sequence, which is directly followed by the malE gene.

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