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pTWIN1 Vector

pTWIN1 is an E. coli expression vector designed for protein purification or for the isolation of proteins with an N-terminal cysteine and/or a C-terminal thioester. A polylinker in the vector is designed for the in-frame fusion of a target gene between the modified Ssp DnaB and Mxe GyrA inteins. The presence of the chitin binding domain from Bacillus circulans facilitates purification.

  • View sequence details
  • Can be used with the IMPACT™ Protein Purification System
  • Double-stranded vector
  • 7,375 base pairs in length
  • Ampicillin resistant
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Catalog # Concentration Size
N6951S 200 µg/ml 10 µg
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  • Product Information

    pTWIN1 is an E. coli expression vector which can be used with the IMPACT™ Kit (NEB #E6901). pTWIN vectors are designed for protein purification or for the isolation of proteins with an N-terminal cysteine and/or a C-terminal thioester (1). A polylinker in the vector is designed for the in-frame fusion of a target gene between the modified Ssp DnaB (2) and Mxe GyrA inteins (3,4). The presence of the chitin binding domain from Bacillus circulans (5,6) facilitates purification. The double-stranded vector is 7,375 base pairs in length.

    DNASU is a central repository for plasmid clones and collections that may also be helpful.

    Reagents Supplied

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
    This product is related to the following categories:
    IMPACT System,
    Bacterial E. coli Protein Expression,
    DNA Plasmids & Substrates Products,
    Protein Expression
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