New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.

pTYB21 Vector

pTYB21 is an E. coli expression N-terminal fusion vector designed for in-frame insertion of a target gene into the polylinker downstream of the intein tag. This allows the N-terminus of the target protein to be fused to the intein tag. The self-cleavage activity of the intein allows the release of the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein.

  • View sequence details
  • Used in the IMPACT™ Protein Purification System
  • Used in conjunction with a C-terminal fusion vector to test which fusion construction (N-terminal or C-terminal) maximizes the expression and yield of a target protein 
  • 7,514 base pairs in length
  • Ampicillin resistant
Learn more about how this product is being used in the Product Citation Tool
2019-20_Catalog_Promo_0519
Catalog # Concentration Size
N6709S 200 µg/ml 10 µg
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