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Home Discontinued Products pKLAC1 Vector

pKLAC1 Vector

Catalog # N3740 was discontinued on January 01, 2013 ;

Product Information
The vector pKLAC1 directs high-level expression of a recombinant protein from the yeast Kluyveromyces lactis and is part of the K. lactis Protein Expression Kit (NEB #E1000). pKLAC1 can be used for either intracellular or secreted protein expression. SacII or BstXI linearized pKLAC1 integrates into the LAC4 locus of the K. lactis genome upon transformation of K. lactis GG799 Competent Cells (NEB #C1001).

Vector pKLAC1 contains the strong K. lactis P_LAC4-PBI promoter (1), DNA encoding the K. lactis α-mating factor (α-MF) secretion domain (for secreted expression), a multiple cloning site (MCS), the K. lactis LAC4 transcription terminator (TT), and a fungal acetamidase selectable marker gene (amdS) expressed from the yeast ADH2 promoter (P_ADH2). An E. coli replication origin (ori) and ampicillin resistance gene (Ap^R) are present for propagation of pKLAC1 in E. coli. NEB 5-alpha Competent E. coli (High Efficiency) (NEB #C2987), NEB 5-alpha Electrocompetent E. coli (NEB #C2989) and NEB 5-alpha Competent E. coli (Subcloning Efficiency) (NEB #C2988) are all recommended for propagation and subcloning of this vector.

Highlights
- P_LAC4-PBI promoter does not express in E. coli allowing toxic genes to be cloned prior to their expression in yeast.
- Convenient MCS lies downstream of α-MF secretion domain and P_LAC4-PBI promoter.
- Acetamidase expression for non-antibiotic selection in K. lactis.
- Ampicillin resistance for propagation in E. coli.
Product Categories:

Discontinued Products
- Properties & Usage
  
  Storage Temperature
  -20°C
  Sequence Files
  Fasta GenBank
- Related Products
  Companion Products
  
  Yeast Carbon Base Medium Powder
  
  K. lactis GG799 Competent Cells
  
  NEB® 5-alpha Competent E. coli (High Efficiency)
  
  NEB® 5-alpha Competent E. coli (Subcloning Efficiency)
  
  NEB® 5-alpha Electrocompetent E. coli
  
  K. lactis Protein Expression Kit
  
  BstXI
  
  SacII
- References
  Colussi, P.A. and Taron, C.H. Unpublished observation
Protocols, Manuals & Usage
- Application Notes
  Coexpression of Multiple Proteins in Kluyveromyces lactis N3740
Tools & Resources
- Web Tools
  DNA Sequences and Maps Tool
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  Licenses
  The vector pKLAC1 is a component of an expression system that was developed from basic research at New England Biolabs, Inc. and DSM Biologics Company B.V. The buyer/user has a non-exclusive sublicense to use this system or any component thereof, including vector pKLAC1, for RESEARCH PURPOSES ONLY. A license to use this system for manufacture of clinical grade material or commercial purposes is available from New England Biolabs, Inc., or DSM Biologics Company B.V.

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