Vector pKLAC1 contains the strong K. lactis PLAC4-PBI promoter (1), DNA encoding the K. lactis α-mating factor (α-MF) secretion domain (for secreted expression), a multiple cloning site (MCS), the K. lactis LAC4 transcription terminator (TT), and a fungal acetamidase selectable marker gene (amdS) expressed from the yeast ADH2 promoter (PADH2). An E. coli replication origin (ori) and ampicillin resistance gene (ApR) are present for propagation of pKLAC1 in E. coli. NEB 5-alpha Competent E. coli (High Efficiency) (NEB #C2987), NEB 5-alpha Electrocompetent E. coli (NEB #C2989) and NEB 5-alpha Competent E. coli (Subcloning Efficiency) (NEB #C2988) are all recommended for propagation and subcloning of this vector.
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