- Size range: 50 bp to 766 bp
- Value pricing
- Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy to identify reference bands
- Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes
How to Prepare and Load a Standard DNA Ladder
The PCR Marker consists of a proprietary plasmid that is digested to completion with appropriate restriction enzymes to yield 5 double-stranded DNA bands suitable for use as molecular weight standards for agarose and acrylamide gel electrophoresis. The digested DNA includes fragments ranging from 50-766 base pairs. The approximate mass of DNA in each of the bands is provided (assuming a 0.3 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.
- Recommended gel percentage range: 1.8-3%
- Optimum separation on 2%
The following reagents are supplied with this product:
Store at (°C) Concentration Gel Loading Dye, Purple (6X), no SDS 25 6 X
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