TriDye™ Ultra Low Range DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing three tracking dyes which serve as visual aids to monitor the progress of migration during native agarose and polyacrylamide gel electrophoresis.
The DNA ladder contains 13 bands ranging from 10 bp to 700 bp, most of which are generated from the digestion of a proprietary plasmid with restriction enzymes. The 50 bp and 300 bp bands have increased intensity to serve as reference bands. The ladder is suitable for use with for use with both native polyacrylamide and agarose gel electrophoresis.
|Gel||Xylene Cyanol||Bromophenol Blue||Orange G|
|4% Agarose||150 bp||20 bp||5 bp|
|5% Agarose||100 bp||15 bp||5 bp|
|6% PAGE||400 bp||50 bp||15 bp|
|10% PAGE||200 bp||30 bp||10 bp|
|4-20% PAGE||300 bp||20 bp||10 bp|
|20% PAGE||200 bp||20 bp||10 bp|
*These migration rates are approximate and variations may occur based on migration conditions (TAE/TBE buffers, buffer pH, voltage etc.).
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
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