Supercoiled DNA Ladder
- Supercoiled molecular weight marker
- Size range: 2 kb to 10 kb
- Convenient reference band at 5 kb
- Supplied with a vial of Gel Loading Dye, Purple (6x), no SDS (NEB #B7025)
- Suitable for 100 gel lanes
How to Prepare and Load a Standard DNA Ladder
The Supercoiled DNA ladder contains 9 proprietary supercoiled plasmids, ranging in size from 2 to 10 kb, that are suitable for use as supercoiled molecular weight standards for agarose electrophoresis. The 5 kb plasmid has an increased intensity to serve as a reference band.
- Recommended gel percentage range: 0.6-1%
- Optimum separation on 0.8%
- Recommended migration conditions: no more than 5V/cm (distance between electrodes), for no more than 60 minutes
Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.
The following reagents are supplied with this product:
Store at (°C) Concentration Gel Loading Dye, Purple (6X), no SDS 25 6X
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