Contains 0.25 ml of 100 mM ultrapure dUTP. dUTP can either be fully substituted for dTTP or added in addition to conventional dNTPs. dUTP can be used with Family A polymerases (such as Taq) or modified Family B polymerases, such as Q5U Hot Start High-Fidelity DNA Polymerase. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.
For a simplified protocol, dUTP can be added to most polymerase reactions at the same concentration as other nucleotides (e.g., a 1:1:1:1:1 dA/dC/dG/dT/dU ratio).
For applications that require other molar ratios (up to and including full substitution of dU for dT), custom nucleotide mixes must be made. We recommend starting with a concentration of 0.2 mM of each dNTP for both Taq and Q5U DNA polymerases.
Ultra purified water as a sodium salt at pH 7.5.
The dUTP Solution is supplied at a concentration of 100 mM (+/- 5 mM) as determined by A260 absorbance.
Can be diluted using sterile distilled water, preferably Milli-Q® water or can be diluted using sterile TE [10 mM Tris-HCl, 1 mM EDTA (pH 7.5)].
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.