Linearized pLox2+ is 3,625 bp in length, with a loxP site approximately 400 bp from each end. Between the loxP sites lie an origin of replication and ampicillin-resistance gene. Recombination between these loxP sites produces a circular (2,787 bp), ampicillin-resistant plasmid (which migrates at approximately 1.7 kb on a 0.8% agarose gel) and one 838 bp DNA fragment.
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