pSV40-GLuc Control Plasmid
- Catalog # N0323 was discontinued on August 02, 2018
The pSV40-GLuc Control Plasmid is a mammalian expression plasmid that constitutively expresses the secreted Gaussia luciferase (GLuc) under the control of the SV40 early promoter.
- Moderate constitutive expression of GLuc
- GLuc can be assayed in the culture media or cell lysate
The pSV40-GLuc Control Plasmid is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive SV40 promoter. Gaussia luciferase (GLuc) is a 19 kDa protein encoded by a “humanized “ sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1) There is a multiple cloning site (MCS) upstream of the SV40 promoter.
- Polylinker MCS: 1–50
- SV40 promoter: 51-246
- GLuc coding: 306–863
- Start codon: 306–308
- Stop codon: 861–863
- Signal peptide: 306–356
- SV40 poly-A site: 890–1111
- SV40 enhancer: 1117–1363
- Bacterial replication ori (pMB1): 2269–1681
- Amp resistance: 3300–2440
Product SourceIsolated from an E. coli strain NEB10b by standard DNA purification procedure.
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