pCLuc-Basic 2 Vector

Features

• Multiple samples can be obtained from the same transfected cells (i.e., before and after experimental treatments or at multiple time points).
• 90–95% of CLuc activity is found in the cell culture medium, with the remaining 5-10% detectable in cell lysates (Figure 1). This allows flexibility when assaying CLuc along with other co-transfected reporters.
• The activity of CLuc is high and the CLuc assay is sensitive enough to detect very small amounts of CLuc enzyme activity.
• CLuc does not use the same substrate as other marine luciferases (e.g. Renilla and Gaussia). Therefore, it is possible to assay both CLuc and GLuc independently in cell culture medium from cells expressing both reporters.
• The pCLuc-Basic 2 Vector can be transfected into cells using any standard transfection protocol and stable cell lines can be established using Neomycin selection.

Application Features

• The pCLuc-Basic 2 Vector can be used to test promoters by cloning promoter element of interest into the MCS upstream of the CLuc reporter gene. For constitutive expression of CLuc, vectors containing promoters are available (See Related Products).
• CLuc can be used as a stand alone reporter or in conjunction with other compatible reporters such as Gaussia Luciferase (GLuc) (2). CLuc and GLuc are ideally suited for co-expression as both are secreted and highly active enzymes providing ease of use and sensitivity (2).

1. Nakajima, Y. et al. (2004). Biosci. Biotechnol. Biochem. 63, 565-570.
2. Wu, C., Suzuki-Ogoh, C. and Ohmiya, Y. (2007). BioTechniques. 42, 290-292.
3. Otsuji, et at (2004). Anal. Biochemistry. 329, 230-237.

• Reporter Systems

• DNA Sequences and Maps Tool

1. Where can I find the sequence of this plasmid?
2. Can I make a stable cell line with pCLuc-Basic 2?
3. Can I transfect this plasmid into mammalian cells?
4. How do I assay for CLuc expression?
5. Can I use assay kits designed for other reporters (Gaussia, Renilla & Firefly luciferases) to assay CLuc activity?
6. Is there another secreted reporter that can be used with CLuc?
7. What is the DNA sequence of this product?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• pCLuc-Basic 2 Vector

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

Licensed under certain patents and patent applications from the National Institute of Advanced Industrial Science and Technology ("AIST") for Research and Development Purposes.

For use of the Biolux Cypridina Luciferase Assay Kit, or associated assay reagents, in human diagnosis and measurement in relation to human health, contact [email protected]