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Histone H2A/H2B Dimer Human, Recombinant
cloned at NEB recombinant

  • Histone H2A combines with Histone H2B to form the H2A/H2B heterodimer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer.
  • Histones are modified by various enzymes and can act as substrates for them. These modifications have been shown to be important in gene regulation. Because the histones are folded with their subunit partners, the dimer may be a better substrate for specific enzymes and modifications.
  • Highly pure, recombinant protein
  • Mass confirmed by ESI-TOF MS
  • Tested for exonuclease, endonuclease and protease contamination

 

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Catalog # Concentration Size
M2508S 20 µM 2 nmol
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  • Product Information

    Protein Sequence: Human Histone H2A Protein Sequence: SGRGK QGGKA RAKAK SRSSR AGLQF PVGRV HRLLR KGNYS ERVGA GAPVY LAAVL EYLTA EILEL AGNAA RDNKK TRIIP RHLQL AIRND EELNK LLGRV TIAQG GVLPN IQAVL LPKKT ESHHK AKGK (Genbank accession number: AAN59960)

    Human Histone H2B Protein Sequence: PEPAK SAPAP KKGSK KAVTK AQKKD GKKRK RSRKE SYSIY VYKVL KQVHP DTGIS SKAMG IMNSF VNDIF ERIAG EASRL AHYNK RSTIT SREIQ TAVRL LLPGE LAKHA VSEGT KAVTK YTSSK(Genbank accession number: AAN59961)

    SDS-PAGE analysis of Histone H2A/H2B Dimer, Recombinant
    SDS-PAGE analysis of Histone H2A/H2B Dimer, Recombinant
    Lane 1&7: NEB Protein Ladder (NEB #P7703)
    Lane 2: Histone H2A (NEB #M2502S)
    Lane 3: Histone H2B (NEB #M2505S)
    Lane 4–6: 2.0, 4.0, 8.0 µg Histone H2A/H2B Dimer

    Product Source

    Purified H2A and H2B (NEB #M2502 and NEB #M2505) were denatured, refolded and the dimer was purified by gel filtration.

    Reagents Supplied

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
    This product is related to the following categories:
    Histones Products
    This product can be used in the following applications:
    Histones & Nucleosome Assembly
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