Pyrophosphatase, Inorganic (yeast)
- Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate
- Enhances yields of RNA in transcription reactions
- Isolated from a recombinant source
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.
A variety of metabolic reactions generate inorganic pyrophosphate as a reaction byproduct. Such reactions are rendered irreversible when the pyrophosphate is degraded by pyrophosphatase (1). RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.
- Isolated from a recombinant source.
Product SourcePPase is prepared from an E. coli strain containing a genetic fusion of the Saccharomyces cerevisiae ppa gene and the gene coding for Mycobacterium xenopi GyrA intein. The final product has been cleaved and purified from the intein fusion partner. Developed by the Biohelix Corporation, and produced at New England Biolabs.
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