NEBNext® Q5® Hot Start HiFi PCR Master Mix
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NEBNext® Q5 Hot Start HiFi PCR Master Mix is the original hot start NEBNext formulation of Q5 High-Fidelity DNA polymerase. This formulation is also included as a component in all of the original NEBNext Ultra kits, for DNA and RNA library preparation.

However, the NEBNext Ultra II Q5 Master Mix (NEB #M0544) is now available, and this more recent hot start formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions. The NEBNext Ultra II Q5 Master Mix is also included as a component in all NEBNext Ultra II kits, for DNA and RNA library preparation.
Learn more about how this product is being used in the Product Citation Tool
2019-20_Catalog_Promo_0519
Catalog # Concentration Size
M0543S Not Applicable 50 reactions
M0543L Not Applicable 250 reactions
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