Thermostable RNase H
Thermostable RNase H specifically recognizes and cleaves the phosphodiester bonds of an RNA strand in an RNA-DNA hybrid while leaving the DNA strand intact. This thermostable nuclease exhibits the same enzymatic properties as E. coli RNase H, but is active at much higher temperatures.
- Recombinant enzyme supplied with 10X Reaction Buffer
- Does not digest single or double-stranded DNA
- Active and stable above 37°C over a broad temperature range, with activity increasing with temperature
- Requires magnesium chloride for activity
- Inactivated with proteinase K treatment or addition of excess EDTA
Thermostable RNase H is an endoribonuclease that is functional at high temperatures and selectively hydrolyzes the phosphodiester bonds of an RNA strand in an RNA:DNA hybrid molecule, while leaving the DNA strand intact. In addition, Thermostable RNase H does not degrade single- or double-stranded RNA or DNA. While the Thermostable and E. coli RNase H homologs both exhibit these same endoribonucleolytic properties, the thermostable enzyme displays activity at higher temperatures with optimal activity above 65°C. These properties allow use in applications where higher assay temperatures are desired.
Product SourceAn E. coli strain carrying a codon optimized plasmid encoding RNase H from the extreme thermophile Thermus thermophilus.
The following reagents are supplied with this product:
Store at (°C) Concentration RNase H Reaction Buffer -20 10 X
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.