Fidelity at its finest.
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
Q5® High-Fidelity DNA Polymerase
is a high-fidelity, thermostable DNA polymerase
with 3´→ 5´ exonuclease activity, fused to a
processivity-enhancing Sso7d domain to support
robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity
DNA Polymerase is ideal for cloning and can
be used for long or difficult amplicons. Q5
High-Fidelity DNA Polymerase is supplied with
an optimized buffer system that allows robust
amplification regardless of GC content. The 5X
Q5 Reaction Buffer contains 2 mM Mg++ at final
(1X) reaction concentrations and is recommended
for most routine applications. For GC-rich targets
(≥ 65% GC), amplification can be improved by
the addition of the 5X Q5 High GC Enhancer. Q5
High-Fidelity DNA Polymerase is unlike typical,
lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at email@example.com or 1-800-632-5227 x 8.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.