OneTaq® DNA Polymerase
The ONE polymerase for your endpoint PCR needs. OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
- 2X higher fidelity than Taq
- Ideal for routine, AT- or GC-rich templates
- Hot start and master mix versions available
How to Amplify GC-rich DNA
5 Tips for Setting Up Your PCR
Why is Tm Important in Primer Design?
Overview of PCR
The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1) . The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.
OneTaq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.
Product SourceAn E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
The following reagents are supplied with this product:
Store at (°C) Concentration OneTaq® Standard Reaction Buffer Pack -20 5 X OneTaq® GC Reaction Buffer Pack -20 5 X OneTaq® High GC Enhancer -20 5 X
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