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In vitro transcription using T7 phage promoter
T7 RNA Polymerase (High Concentration) is offered at a 20-fold higher concentration than our standard T7 RNA Polymerase (NEB #M0251) and is ideal for experienced users interested in building and optimizing their own in vitro transcription reactions. The enzyme is accompanied by RNA Polymerase Reaction Buffer and magnesium chloride solution to further enable testing of different conditions such as nucleotide concentrations.
For standard RNA synthesis and high yield reactions, we recommend our T7 RNA Polymerase (NEB #M0251) and HiScribe™ kits. The HiScribe kits are specifically designed to produce high quality, high yield RNA in a short time with minimal optimization.
In vitro transcription involves multiple components in addition to T7 RNA Polymerase including ribonucleotides, inorganic pyrophosphatase and RNase Inhibitor, which can be purchased separately. Please refer to the Related Products section for details. More information regarding transcription optimization can be obtained from the following article.
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. The 99 kD enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T7 promoters. RNA produced using the T7 RNA Polymerase is suitable for many applications in research and biotechnology.
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