- DNA specific exonuclease
- Cleaves linear single-stranded DNA in both the 3' to 5' and 5' to 3' directions
- Preferred substrate is linear single-stranded DNA
Exonuclease VII is ideal for:
- Removal of primers with or without 3' or 5' terminal phosphorothioate bonds
- Mapping positions of introns in genomic DNA
- Removal of single-stranded DNA, leaving behind the double-stranded DNA in a sample
Exonuclease VII, (Exo VII) derived from E. coli, cleaves single-stranded DNA (ssDNA) from both 5´→3´ and 3´→5´ direction. This enzyme is not active on linear or circular dsDNA (1,2). It is useful for removal of single stranded oligonucleotide primers (3) from a completed PCR reaction when different primers are required for subsequent PCR reactions. Digestion of ssDNA by Exonuclease VII is metal-independent.
Product SourceAn E. coli strain that carries cloned Exonuclease VII (XseA and XseB) genes from E. coli.
The following reagents are supplied with this product:
Store at (°C) Concentration Exonuclease VII Reaction Buffer -20 5X
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