Bst 3.0 DNA Polymerase
Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
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Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity. Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
Fast, single-enzyme RT-LAMP can be performed using Bst 3.0
RT-LAMP was performed using indicated DNA polymerase and Jurkat total RNA and primers for two genes (ACTB, left; HMBS2, right). Fastest results were observed with a 2-enzyme system, Bst 2.0 and WarmStart RTx, but robust amplification was also observed using Bst 3.0 without additional RT. Bst LF, Bst 2.0 and competitor enzymes showed highly variable performance, with slow threshold times or reaction failure on one of the two targets.Product Source
An E. coli strain that carries the engineered Bst 3.0 gene.Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) Concentration Isothermal Amplification Buffer II Pack -20 10 X Magnesium Sulfate (MgSO4) Solution -20 100 mM - Product Categories:
- Isothermal Amplification & Strand Displacement Products
- Applications:
- Whole Genome Amplification,
- Isothermal Amplification
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