Bst 3.0 DNA Polymerase
Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.
Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
- Fused to novel nucleic acid binding domain for enhanced performance
- Optimized for Loop-Mediated Isothermal DNA Amplification (LAMP)
- Simplified reaction setup: single-enzyme RT-LAMP reaction
- High reverse transcriptase activity up to 72°C
- Offers fast polymerization rates and robust performance even in presence of inhibitors, including dUTP
- Learn more about LAMP and other isothermal amplification methods
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Product Information
Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered and fused to a novel nucleic acid binding domain for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity. Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors, including dUTP and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.An example of the utility and speed offered by Bst 3.0 DNA Polymerase can be seen in this publication that explores the development of a novel digital LAMP assay: Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples.
Fast, single-enzyme RT-LAMP can be performed using Bst 3.0
RT-LAMP was performed using indicated DNA polymerase and Jurkat total RNA and primers for two genes (ACTB, left; HMBS2, right). Fastest results were observed with a 2-enzyme system, Bst 2.0 and WarmStart RTx, but robust amplification was also observed using Bst 3.0 without additional RT. Bst LF, Bst 2.0 and competitor enzymes showed highly variable performance, with slow threshold times or reaction failure on one of the two targets.Product Source
An E. coli strain that carries the engineered Bst 3.0 gene.Reagents Supplied
The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration - This product is related to the following categories:
- Isothermal Amplification & Strand Displacement Products
- This product can be used in the following applications:
- Whole Genome Amplification,
- Loop-Mediated Isothermal Amplification,
- Isothermal Amplification
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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Citations & Technical Literature
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Citations
Additional Citations
- Lee D, Shin Y, Chung S, Hwang KS, Yoon DS, Lee JH (2016) Simple and Highly Sensitive Molecular Diagnosis of Zika Virus by Lateral Flow Assays. Anal Chem; PubMedID: 28193014, DOI: 10.1021/acs.analchem.6b03460
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Citations
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