Bst 3.0 DNA Polymerase

Application Features

• Isothermal amplification (LAMP, RT-LAMP)
• Applications requiring strand-displacement DNA synthesis
• Reverse transcription at elevated temperature (to 72°C)
• Amplification in the presence of impure samples or amplification inhibitors

Companion Products

• Isothermal Amplification Buffer II Pack
• Magnesium Sulfate (MgSO₄) Solution
• WarmStart® RTx Reverse Transcriptase
• Deoxynucleotide (dNTP) Solution Set
• Deoxynucleotide (dNTP) Solution Mix

1. Bst 3.0 DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.
2. Reaction temperatures above 72°C are not recommended.
3. Bst 3.0 DNA Polymerase cannot be used for PCR or reactions requiring thermal denaturation.

1. Typical LAMP Protocol (M0374)

1. What is the difference between Bst DNA Polymerase, Large Fragment, Bst 2.0, and Bst 3.0 DNA Polymerase?
2. Why would I use Bst 3.0 DNA Polymerase?
3. Can Bst 3.0 DNA Polymerase be used in other NEBuffers?
4. How active is Bst 3.0 at other temperatures?
5. Does Bst 3.0 DNA polymerase incorporate dUTP?
6. Does Bst 3.0 DNA polymerase have reverse transcriptase activity?
7. Can Bst 3.0 DNA Polymerase be used to blunt DNA?
8. Can Bst 3.0 DNA Polymerase be used to fill in 3' overhangs?
9. Can Bst 3.0 DNA Polymerase be used to remove 5' overhangs?
10. Can Bst 3.0 DNA Polymerase be heat inactivated?
11. Are NEB DNA Polymerases supplied with dNTPs?
12. What are the main causes of reaction failure using Bst 3.0 DNA Polymerase?
13. Does Bst 3.0 DNA Polymerase have an active 3'→5' proofreading exonuclease?
14. Can Bst 3.0 DNA Polymerase be used in applications requiring thermal cycling?
15. Can Bst 3.0 DNA Polymerase initiate at a nick in the DNA?
16. Can Bst 3.0 DNA Polymerase be used in labeling reactions and partial fill in reactions?
17. Can Bst 3.0 DNA Polymerase be diluted?
18. When I thaw Isothermal Amplification Buffer II I see a lot of white precipitate, is this normal?
19. When should Bst 3.0 DNA Polymerase be the enzyme of choice?
20. How do I reduce non-template amplification (NTC) in LAMP reactions with Bst 3.0?
21. How do I use Antarctic Thermolabile UDG for carryover prevention in LAMP reactions?
22. Does NEB have a master mix for LAMP or RT-LAMP reactions?

Product Citation Tool

Additional Citations

• Lee D, Shin Y, Chung S, Hwang KS, Yoon DS, Lee JH (2016) Simple and Highly Sensitive Molecular Diagnosis of Zika Virus by Lateral Flow Assays. Anal Chem.; PubMedID: 28193014, DOI: 10.1021/acs.analchem.6b03460

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0374S_L_v1
• M0374M_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0374M_v1_0071507
• M0374S_L_v1_0071507
• M0374S_L_v1_0071512
• M0374M_v1_0071512
• M0374S_L_v1_0071605
• M0374M_v1_0071605
• M0374M_v1_0071612
• M0374M_v1_0071706
• M0374M_v1_0071709
• M0374S_L_v1_0071707
• M0374S_L_v1_0071712
• M0374M_v1_0071712
• M0374L_v1_10012706
• M0374S_v1_10013475
• M0374L_v1_10031830
• M0374S_v1_10031748
• M0374M_v1_10034502
• M0374S_v1_10035264
• M0374L_v1_10040162
• M0374M_v1_10047128
• M0374S_v1_10047129
• M0374L_v1_10047130
• M0374L_v1_10055245
• M0374S_v1_10051175
• M0374S_v1_10055150
• M0374S_v1_10058030
• M0374M_v1_10061507
• M0374L_v1_10067887
• M0374S_v1_10062533
• M0374L_v2_10069640
• M0374L_v2_10071207
• M0374L_v2_10071215
• M0374L_v2_10072241
• M0374L_v2_10072250
• M0374S_v2_10071217
• M0374M_v2_10072234
• M0374S_v2_10073912
• M0374L_v2_10073509

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Bst 3.0 DNA Polymerase
• Isothermal Amplification Buffer II Pack
• Magnesium Sulfate (MgSO₄) Solution

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

The purchase of NEB Bst products conveys to the purchaser the limited, nontransferable right to use the purchased products to perform loop-mediated isothermal amplification ("LAMP") for research use only. LAMP is a patented technology belonging to Eiken Chemical Co., Ltd. and any use other than research may require a license from Eiken Chemical Co., Ltd. A patent is pending for NEB's Bst DNA Polymerase.