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Shrimp Alkaline Phosphatase (rSAP)
neb31 recombinant 37 65 Heat

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

Shrimp Alkaline Phosphatase (rSAP) is a heat labile alkaline phosphatase purified from a recombinant source.

  • Rapid and irreversible heat inactivation eliminates unwanted activity
  • Improved storage stability versus native enzyme
  • Faster reaction setup (no supplemental additives like zinc required)
  • Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
  • Less enzyme required (high specific activity), resulting in a lower cost per reaction
  • No need for multiple phosphatases (rSAP removes 5´- and 3´- phosphates from DNA, RNA and dNTPs )
  • Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis
  • Recombinant for purity, consistency and value
Note: See also Exo-CIP™ Rapid PCR Cleanup Kit (NEB #E1050).
Catalog # Concentration Size
M0371S 1,000 units/ml 500 units
M0371L 1,000 units/ml 2,500 units
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