mRNA Cap 2´-O-Methyltransferase
mRNA Cap 2´-O-Methyltransferase adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA.
- mRNA cap-1 structure may improve expression during microinjection and transfection experiments
- Isolated from a recombinant source
- Tested for the absence of endonucleases, exonucleases, RNases
Avoiding RNase Contamination
mRNA Cap 2´-O-Methyltransferase adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure.
The cap-1 structure has been reported to enhance mRNA translation efficiency (1) and hence may help improve expression in mRNA transfection and microinjection experiments.
mRNA Cap 2´-O-Methyltransferase specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5´ end (2,3). Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme (NEB #M2080 ).
The reagents provided in this pack can be used to methylate up to 400 μg of capped RNA.
Product SourceAn E. coli strain that carries the gene for the Vaccinia mRNA Cap 2´-O-Methyltransferase.
The following reagents are supplied with this product:
Store at (°C) Concentration Capping Buffer -20 10 X S-adenosylmethionine (SAM) -20 32 mM
- RNA Modification
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