Crimson Taq DNA Polymerase with (Mg-free) Buffer

Features

• Robust and reliable reactions
• Samples can be loaded directly onto a gel
• Ideal for high throughput applications

Application Features

• PCR
• Primer Extension
• Colony PCR
• High-throughput PCR

Companion Products

• Taq 5X Master Mix
• Taq 2X Master Mix
• Taq PCR Kit
• Quick-Load® Taq 2X Master Mix
• Deoxynucleotide (dNTP) Solution Set
• Deoxynucleotide (dNTP) Solution Mix

Materials Sold Separately

• Crimson Taq (Mg-free) Reaction Buffer Pack
• Magnesium Chloride (MgCl₂) Solution

1. 5'→3' flap endonuclease destroys displaced strand.

1. Chien, A., Edgar, D.B. and Trela, J.M. (1776). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

1. PCR Protocol for Crimson Taq DNA Polymerase with (Mg-free) Buffer (M0325)

• Guidelines for PCR Optimization with Taq DNA Polymerase
• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• DNA Polymerase Selection Chart
• Taq Buffer Selection Chart
• Thermophilic DNA Polymerases

1. What are the advantages or disadvantages of Crimson Taq DNA Polymerase?
2. How do I remove the dye and Dextran from my PCR reactions using Crimson Taq (Mg-free)Reaction Buffer?
3. How long is the Crimson Taq Reaction buffer stable under sub-optimal storage conditions?
4. Can Taq DNA Polymerase be used in other buffers?
5. When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
6. Why is the product a smear when visualized on an agarose gel?
7. Why is there no product when visualized on an agarose gel?
8. The product sequence doesn't completely match the expected sequence. How can this result be improved?
9. Does the presence of Ca²⁺ inhibit PCR?
10. How should I set up an amplification reaction using Crimson Taq DNA Polymerase?
11. What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
12. Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers?
13. Can Taq DNA Polymerase be used for nick translation?
14. Does the 5X Crimson Taq Reaction Buffer offer amplification efficiency similar to that of Standard Taq Reaction Buffer or ThermoPol Reaction Buffer?

• PCR Troubleshooting Guide
• Taq PCR Kit Troubleshooting Guide

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Crimson Taq DNA Polymerase with (Mg-free) Buffer

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While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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